Description
The Salivary DHEA-S EIA Kit is based on the competition principal and microplate separation. An unknown amount of DHEA-S present in a saliva sample and a fixed amount of DHEA-S conjugated to horse radish peroxidase DHEA-S-HRP compete for binding sites with a rabbit polyclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away. Enzyme substrate solution is then added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of DHEA-S-HRP detected is inversely proportional to the amount of DHEA-S in a sample